منابع مشابه
O-3: Identification and Characterization of Repopulating Spermatogonial Stem Cells from The Adult Human Testis
Background: This study was conducted to identify and characterize repopulating spermatogonial stem cells (SSCs) in the adult human testes. Materials and Methods: Testes biopsies from obstructive azoospermic patients and normal segments of human testicular tissue were used. Flow cytometry, real time PCR and immunohistochemical analysis were performed. Purified human spermatogonia were transplant...
متن کاملId4 Marks Spermatogonial Stem Cells in the Mouse Testis
Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing ...
متن کاملSpermatogonial Stem Cells (SSCs) in Buffalo (Bubalus bubalis) Testis
BACKGROUND Water buffalo is an economically important livestock species and about half of its total world population exists in India. Development of stem cell technology in buffalo can find application in targeted genetic modification of this species. Testis has emerged as a source of pluripotent stem cells in mice and human; however, not much information is available in buffalo. OBJECTIVES A...
متن کاملEffects of nano-selenium on mRNA expression of markers for spermatogonial stem cells in the testis of broiler breeder males
Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of Nano-Selenium (Nano-Se) on expression of molecular markers of spermatogon...
متن کاملThe molecular signature of spermatogonial stem/progenitor cells in the 6-day-old mouse testis.
To characterize the molecular phenotype of spermatogonial stem cells (SSCs), we examined genes that are differentially expressed in the stem/progenitor spermatogonia compared to nonstem spermatogonia. We isolated type A spermatogonia (stem and nonstem type A) from 6-day-old mice using sedimentation velocity at unit gravity and further selected the stem/progenitor cell subpopulation by magnetic ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.24.11287